Metabolomics Platform & Mass Spectrometry Services

Inmet

Inmet applies Metabolic Engineering techniques to efficiently transform agro-industrial by-products in bioplastics, biodiesel and compounds of agricultural value.
In this frame, we optimized several technical applications based on Mass Spectrometry in order to monitor processes, raw material quality control, and quantification of bio-product or metabolic intermediaries produced by microorganisms used as bio-factories.
Also, our platform provides technical advices and development of analytical techniques as services, both, to external clients (privates & publics) and to Bioceres Holding’ companies.

Capabilities & Services:

Gas Chromatography + Mass Spectrometry (GC-MS)

Ultra-high-performance liquid chromatography (UHPLC) + Tandem Mass Spectrometry (MS/MS) / UV-Visible Spectroscopy / Refractive Index Detector (RI)

NMR-1D-1H analysis

Gas Chromatography + Mass Spectrometry (GC-MS)

Basis

Gas chromatography coupled with mass spectrometry (GC-MS) is a powerful analytical technique that combines GC separation capability with selectivity and sensibility of mass detector. This “blend” allows identify, isolate, analyze, and quantify volatile and semi-volatile organic compounds in complex mixes with a high degree of efficiency.

Applications

  • Qualitative (by relative percentages) & quantitative (by performing calibration curve with a commercial standard) determinations;
  • Quali-quantitative analysis of volatile and semi-volatile compounds in essential oils of medicinal plants and spices;
  • Determination bacterial lipid profiles (sterols, fatty acids);
  • Aliphatic hydrocarbons, aromatic hydrocarbons, poly-aromatic hydrocarbons (PAHs), FAMEs;
  • We have experience on Headspace GC-MS: technique used for determination of volatile organic compounds from solid or liquid matrixes;
  • Developments of analytic methods upon request.

Sample types

We adapt to any kind of matrix.

Ultra-high-performance liquid chromatography (UHPLC) + Tandem Mass Spectrometry (MS/MS) / UV-Visible Spectroscopy / Refractive Index Detector (RI)

Basis

Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is a very powerful analytical technique. It combines the ability of physical separation that offers liquid chromatography with the sensibility and selectivity of tandem mass detector. This methodology enables the isolation, detection, classification and quantification of specific organic compounds in very complex mixtures or matrixes.

Applications

  • Pre-analysis service: solid-phase extractions (SPE) (C18 / silica columns), liquid-liquid extractions with solvents, filtrations, drying by inert N2 flow or freeze-drying over different matrixes, before LC analysis;
  • Direct infusions, with syringe bomb, of pure compounds;
  • Profile of secondary metabolites on plant extracts, e.g. phenolic compounds;
  • Determination of bacterial fermentation products by using HPLC-UV-RI;
  • Quantification of carbon sources (sugars / alcohols) in culture media by using the HPLC-RI (Refractive Index) technique;
  • Quantification of intracellular metabolites of bacteria, e.g. CoAs pools, phosphorylated metabolic intermediaries;
  • Molecular weights determination of synthetic / natural polymers by using Gel Permeation Chromatography (GPC) method with chloroform as mobile phase. Polydispersity Index (PD), Weight Average Molecular Weight (Mw) and Number Average Molecular Weight (Mn) calculations.

Sample types

We adapt to any kind of matrix.

NMR-1D-1H analysis

  • Analysis of samples (in chloroform or aqueous solutions): Recording of 1H unidimensional spectra, spectra interpretation and identification of protons resonances.

Human Resources

Gonzalo R. Lamberto

Gonzalo has a degree in Biotechnology (Universidad Nacional de Rosario – 2005) and a Ph.D. in Biological Science (Universidad Nacional de Rosario – 2012).

He has broad experience in different fields of knowledge such as Biophysics, Molecular Biology, and Biochemistry. Gonzalo is an expert in analytical techniques studying biomolecules like UV-Visible Spectroscopy, Nuclear Magnetic Resonance (NMR) and HPLC-MS/MS.

In 2012 he joined Inmet with the principal focus on mounting the Metabolomics and Mass Spectrometry Platform of the company. Now, he is the head of this area that has implemented more than 50 methodological developments for detection and quantification of different metabolites and has offered more than 250 R&D Services (Internal & External). 

Mariángeles Arroyo

Mariángeles is a Bromatology Technician (Centro Rosarino de Estudios Avanzados 2003) and joined the Metabolomics Platform in April 2015 as Assistant Technician. Her position is dedicated to the development and application of diverse analytical techniques using Gas Chromatography coupled to Mass Spectrometry equipment (GC-MS).

For Mass Spectrometry Services::
Gonzalo R. Lamberto, PhD
MS Services Supervisor
[email protected]

Contact Us

8 + 2 =

Inmet S.A.

+54 341 4861121 (Management)
+54 341 4861120 (Services)
Ocampo 210 bis, Predio CCT Rosario (2000) Rosario, Santa Fe, Argentina

For more information about our services or to set up an appointment in order to discuss your project of industrial residues bio-transformation, you do not hesitate in contacting us for any questions.